مطالعه تاثیر ضدآنتی‌اکسیدانی عصاره برگ رزماری و میوه توت‌فرنگی بر روی سلول‌های سرطانی معده

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دکتری، گروه شیمی، دانشکده علوم پایه، موسسه آموزش عالی مهرآیین، بندرانزلی، ایران

2 دکتری، گروه زیست‌شناسی، دانشکده علوم پایه، واحد ایذه، دانشگاه آزاد اسلامی، ایذه، ایران

3 کارشناسی، گروه زیست‌شناسی، دانشکده علوم پایه، واحد تالش، دانشگاه پیام نور، تالش، ایران

چکیده

هدف: سرطان معده یکی از شایع‌ترین سرطان‌ها در جهان است که سبب مرگ و میرهای زیای در میان انسان‌ها می‌شود. هدف از انجام این پژوهش بررسی تاثیر ضد آنتی‌اکسیدانی عصاره برگ رزماری و میوه توت‌فرنگی بر روی سلول‌های سرطانی معده است.
مواد و روش‌ها: پس از تهیه دو ماده گیاهی و عصاره‌گیری از آنها، اقدام به اندازه‌گیری توان آنتی اکسیدانی احیاء آهن(FRAP)، تاثیر سمّیت دو عصاره گیاهی مورد استفاده بر رشد و تکثیر سلول‌های سرطانی و فیبروبلاستی در غلظت‌های 5/0، 1، 5/1 و 2 میلی‌گرم بر میلی‌لیتر با روش MMT و بررسی میزان IC50 در دو عصاره گیاهی مورد سنجش برای زمان‌های 24، 48، 72 و 96 ساعت شد. از آزمون تحلیل واریانس یک راهه با سطح معناداری 05/0 برای آنالیز نتایج به دست آمده استفاده گردید.
نتایج: نتایج نشان داد که بالاترین میزان FRAP برای عصاره میوه توت‌فرنگی و برگ رزماری در حلال آب به ترتیب با مقادیر 08/0±68/1 و 15/0±94/0 است. نتایج به دست آمده نشان داد که مقدار
2 میلی‌گرم بر میلی‌لیتر برای عصاره برگ رزماری و میوه توت‌فرنگی بهترین اثر را برای جلوگیری از رشد و تکثیر سلول‌های سرطانی و فیبروبلاستی دارد. مقادیر حاصل برای IC50 نشان داد که مقدار غلظت مورد نیاز برای مهار 50 درصد فعالیت آنتی اکسیدانی برای دو عصاره در زمان 96 ساعت دارای کم‌ترین مقدار است.
نتیجه‌گیری: هر دو عصاره دارای اثرات ضد سرطانی بر روی رده سلولی AGS در سرطان معده هستند که میوه توت‌فرنگی داری اثر مناسب‌تری نسبت به برگ رزماری است.
 

کلیدواژه‌ها


عنوان مقاله [English]

Study of antioxidant effect of rosemary leaf extract (Rosmarinus officinalis) and strawberry fruit extract (Fragaria vesca) on stomach cancer cells

نویسندگان [English]

  • Mehdad Enkari 1
  • Samira Goodarzi 2
  • Kiana Ansari 3
1 PhD. Department of Chemistry, Faculty of Basic Sciences, Mehraein Institute of Higher Education, Bandar Anzali, Iran
2 PhD. Department of Biology, Faculty of Basic Sciences, Izeh Branch, Islamic Azad University, Izeh, Iran.
3 Masters, Department of Biology, Faculty of Basic Sciences, Talesh Branch, Payam Noor University, Talesh, Iran.
چکیده [English]

Purpose and Problem: Stomach cancer is one of the most common cancers in the world that causes many deaths among humans. The aim of this study was to investigate the antioxidant effect of rosemary leaf extract (Rosmarinus officinalis) and strawberry (Fragaria vesca) on stomach cancer cells.
Method: After preparing two plant substances and extracting them, measure the antioxidant power of iron regeneration (FRAP), the toxicity of the two plant extracts used on the growth and proliferation of cancer cells and fibroblasts in concentrations. 0.5, 1, 1.5 and 2 mg/ml were measured by MMT method and IC50 was measured in two plant extracts for 24, 48, 72 and 96 hours. One-way analysis of variance with a significance level of 0.05 was used to analyze the results.
Results: The results showed that the highest FRAP levels for strawberry fruit and rosemary leaf extracts in water solvent were 1.68 ± 0.08 and 0.94± 0.15, respectively. The results showed that 2mg /ml for rosemary leaf extract and strawberry fruit has the best effect to prevent the growth and proliferation of cancer cells and fibroblasts. The values obtained for IC50 showed that the concentration required to inhibit 50% of the antioxidant activity for the two extracts at 96 h was the lowest.
Conclusion: The results showed that both extracts have anti-cancer effects on AGS cell line in Stomach cancer that strawberry fruit has a more suitable effect than rosemary leaf.
 

کلیدواژه‌ها [English]

  • Anti-cancer effects
  • Stomach Cancer
  • Strawberry fruit extract
  • Rosemary leaf extract
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